BestScoep B-SIM298 Structured Illumination Fluorescence Microscope for laboratory

• Customization: Available
• Warranty: 3 Years
• Magnification: 40-1000x
• Model NO.: B-SIM298
• Type: Fluorescent
• Number of Cylinder: Binoculars
• Mobility: Desktop
• Stereoscopic Effect: Without Stereoscopic Effect
• Kind of Light Source: Fluorescence
• Shape: Cylindrical Shaped Lens
• Usage: Teaching, Research
• Principle: Optics
• Principle of Optics: Infinity Color Correction Optical System
• Viewing Head: 20-45 degree tilting binocular tube
• Eyepiece: High eye point wide field plan eyepiece PL10X/22mm
• Objective: 4X, 10X, 20X, 40X, 60X
• Nosepiece: Electric bright field sextuple nosepiece
• Stage: 300mmX240mm
• Illumination: Koehler transmission illuminator
• Condenser: Electric septuple condenser, NA 0.55, WD=27mm
• Transport Package: Strong Carton with Polyfoam Protection
• Trademark: BestScope
• Origin: Beijing
• Production Capacity: 1000PCS/Month

Product Description

         

Introduction

New designed B-SIM298 Fluorescence microscope integrates observation and analysis. Automated control of imaging process, excellent for live cell imaging. B-SIM298 has powerful expansion functions, providing a more complete solution for life science research.

Features

1. High optical resolution

X/Y axis resolution ratio: 240nm; Z-axis resolution ratio: 600nm.
 

2. High sensitivity backlit sCMOS camera

High sensitivity backlit sCMOS camera, with a frame rate of up to 100 frames per second and a quantum efficiency of up to 95% at 2048x2048 pixels, with a 6.5um pixel size.
 

3. Illumination

4-wavelength LED light source, long life, high brightness, low light toxicity, high uniformity lighting solutions.
 

4.Software

• Hardware control: 12-axis fully electric control, camera control, supports third-party light source, electric stage and other peripheral control.
• Image acquisition: x, y, z, λ, t, n, l, seven-dimensional acquisition, multi-dimensional, full process automated control.
• Image processing: 3D reconstruction and display, co-localization processing, co-localization linkage, image brightness, contrast, threshold processing, image flipping, mirroring, background removal, dynamic image generation, stack processing and ROI processing.
• Image analysis: distance, perimeter, area, roundness, maximum and minimum gray scale, and other parameter analysis, co-localization analysis, cell counting, particle counting, protein tracking, subpopulation analysis and cell cycle analysis.

• Five-dimensional sequence acquisition of x, y, z, λ , t, realizes automatic acquisition control.
• Graphical interaction: switch optical port, filter block, objectives with one-click and changing observation mode.
• Electric focusing, electric stage, electric fluorescence filter block, electric condenser, electric light source control, electric light path switching, realizing fully electric 12-axis control.
• Real-time optical density measurement, real-time global histogram display.
• Graphical focus control, focus position memory, automatic focus control.
• Customize 1-8 channel acquisition, real-time pseudo-color display, arbitrary multi-channel overlay, and real-time display of multi-channel overlay map.

• Real-time display of comparison before and after image processing
• Record image information in detail, including detailed parameters such as acquisition channel, objectives, exposure time, etc.
• Real-time image preview
• 3D image reconstruction

Application

BS-2064 series microscopes are ideal instrument in biological, histological, pathological, bacteriology, immunizations and pharmacy field and can be widely used in medical and sanitary establishments, laboratories, institutes, academic laboratories, clinics, medical colleges and universities.

Specification

Item	Specification		B-SIM298
Optical System	Infinity Color Correction Optical System		*
Viewing Head	High eye point wide field plan eyepiece PL10X/22mm, with adjustable diopter		*
Eyepiece	20-45 degree tilting binocular tube, interpupillary distance: 50-76mm		*
Objectives	Long Working Distance Plan Semi- Apochromatic Objectives	4X/NA=0.13, WD=17mm	○
		10X/NA=0.3, WD=8.8mm	○
		20X/NA=0.45, WD=6.5-7.6mm, coverslip thickness: 0-2mm	*
		40X/NA=0.6, WD=2.85-4.05mm, coverslip thickness: 0-2mm	*
		60X/NA=0.7, WD=1.42-2.1mm, coverslip thickness: 0-1.3mm	○
	Infinity Plan Semi-Apochromatic Objectives	4X/NA=0.16, WD=12.8mm	*
	Infinity Plan Super Apochromatic Objectives	10X/NA=0.4, WD=3.1mm	*
		20X/NA=0.8, WD=0.6mm	○
		40X/NA=0.95, WD=0.18mm	○
		(Oil) 60X/NA=1.42, WD=0.17mm	*
		(Oil) 100X/NA=1.45, WD=0.13mm	○
Microscope Body & Nosepiece	Low position coarse and fine coaxial electric focusing mechanism, range: 10.5mm, precision: 1μm. Built-in electric upper camera port, splitting ratio: 100:0 / 0:100. Built-in electric left camera port, splitting ratio: 0:100 / 50:50 / 100:0, dual optical path, with fluorescent light barrier. Electric bright field sextuple nosepiece with DIC slot and upper optical port CTV adapter.		*
	Base mounting bracket		○
	Right camera port, splitting ratio: 100:0 / 0:100, field of view: 16mm. Built-in 1X CTV, C-mount adapter		○
Illumination	Pillar tilt mechanism, Koehler transmission illuminator, adjustable condenser holder with 65mm stroke. 4 filters holders with LBD, Green filter, Neutral filter for halogen models or Neutral filter for LED models		*
	12V/100W halogen illumination, filament center preset		○
	12V/100W halogen lamp		○
	10W cool color LED light illumination, color temperature 5000K		*
Condenser & Iris	Electric septuple condenser, NA 0.55, WD=27mm. 3 holds for Φ30mm (phase contrast), 4 holds for Φ38mm (DIC), support for bright field/phase contrast /DIC (with polarizing kit)		*
	Super long working distance manual condenser with 5 holes, NA 0.3, WD=73mm, support for 4X-60X phase contrast, simple polarizing observation and 10X-40X relief phase contrast observation.		○
	Cable 100cm		*
DIC	Transmitted DIC kit		*
	10X transmitted DIC ring (for BSIM2980022)		*
	20X transmitted DIC ring (for BSIM2980022)		*
	40X/60X transmitted DIC ring (for BSIM2980022)		*
	Analyzer kit (for BSIM2980022)		*
Fluorescent Module	Fluorescence attachment with 8 holes, with electric shutter		*
	Cable 30cm, connecting the fluorescence attachment to the frame		*
	Dust cap		*
	UV fluorescence filter house, EX: AT375/28X, 25mm, BS:AT415DC, 25.5*36*1mm, EM:AT460/50M, 25mm		*
	B fluorescence filter house, EX: AT480/30X, 25mm, BS: AT505DC, 25.5*36*1mm, EM: AT535/40M, 25mm		*
	G fluorescence filter house, EX: AT560/40X, 25mm, BS: AT600DC, 25.5*36*1mm, EM: AT635/60M, 25mm		*
	G fluorescence filter house, EX: AT620/50X, 25mm, BS: AT655DC, 25.5*36*1mm, EM: AT690/50M,25mm		*
	LED light source: 380/475/550/630 four wavelengths, four independent lamp beads, high-power, long-life LED light source.		*
Stage	Manual mechanical stage, size: 300mm(X)*240mm(Y), moving range: 135mm(X)*85mm(Y), stage thickness: 30mm. Right universal handle, X/Y axis limitable and lockable, moving range 50mm * 50mm after locked; with pressure clap for holding slices and culture flasks, with Φ110mm replaceable disc (inner Φ30), with metal stage plate with waist shaped hole.		*
Electric Control Box & PC	Electric control box, input voltage 90-265VAC wide voltage, output 12V100W or 12V10W.Digitally adjustable output voltage through CAN, in addition to three outputs of 24V5A/15V5A/5V5A, equipped with forced air cooling, including one 3C power cord.		*
	DB26 Cable 200cm, connecting the electric control box to the frame		*
	DELL 3020T computer: I7-12700 32GB RAM 1TB solid state, host RTX3060TI+8G independent display, Dell 34 display, curved USB-C display iS3423DWC, WIN10		*
	DB9 Cable 200cm, connecting the PC to the electric control box		*
	USB-CAN card. When the customer purchases computer by themselves and requires computer to control microscope electric control, it must be paired.		*
Structured Light Components	Grid size: 40um. Light transmission range: 400-750nm; liquid core fiber: core diameter: 3mm. Numerical aperture: 0.5. Transmission range: 240-740nm		*
Camera	sCMOS back-illuminated black and white camera. Global pixels: 2048*2048, 4 million effective physical pixels. Pixel size: 6.5μm*6.5μm. Chip size: 13.3mm*13.3mm. Quantum efficiency: 95% @ 600nm. Frame rate: 100fps@CameraLink, 40fps@USB 3.0. Full well capacity: typical: 45 ke-. Dynamic range: 90dB. Readout noise: CMS: 1.1E-Median, 1.2E-RMS.  Exposure time: 6.6μs-10s.Refrigeration method: air cooling, water cooling. Dark current: air cooling: 0.15e-/pixel/s@-15ºC, water cooling: 0.10e-/pixel/s@-25ºC. Bit depth: 11bit, 12bit, 16bit.		*
Hardware Control	Hardware control: Automatically control the structured light lighting system. Image acquisition: exposure time control, gain control, threshold control, gamma value control, image pixel number control, x, y, z, λ, t five-dimensional acquisition, custom xyz, xyt, xyzt, xyλ, xyλt, xyzλt, multiple collection modes. Start recording with one click. Supports real-time pseudo-color annotation and real-time optical density measurement. Image processing: 3D reconstruction and display, image flipping, mirroring, background removal, dynamic image generation, stack processing, ROI processing. Image analysis: perimeter, area, roundness, maximum gray level, minimum gray level and other parameter analysis, co-localization analysis, cell counting, point counting, protein tracking, subpopulation analysis.		*
Telescope	Telescope (Φ30)		○
Adapter	1X C-mount adapter, adjustable focus		*
Other Accessories	Fluorescent free oil 30ml		*
	Internal hexagonal Spanner M3 for phase contrast adjusting screw		*
	Internal hexagonal Spanner M4		*
	Internal hexagonal Spanner M5		*

Note: * Standard Outfit, ○Optional

Accessories

1. Infinity plan super apochromatic objectives

Sample Image